Menyhárt J, Marcsek Z, Gróf J
Acta Physiol Acad Sci Hung. 1981;58(2):119-29.
Aqueous extracts of resting (C) and 30 h regenerating (P) rat livers were partially purified by a combination of ion exchange-, Sephadex- and paper chromatographic techniques. It was demonstrated that a limited number of the semipurified chromatographic fractions inhibited or stimulated both DNA synthesis and cell proliferation, the effect being partially determined by C or P liver origin of the fractions. It was also shown that biological activities were mediated by hepatic peptides of middle molecular size in the semipurified fractions. It is suggested that regulation of hepatocyte proliferation might be under a dual control operating through an interplay of stimulatory and inhibitory peptides, the final outcome determined by their interaction.
通过离子交换、葡聚糖凝胶和纸色谱技术相结合的方法，对静止（C）和再生30小时（P）的大鼠肝脏水提取物进行了部分纯化。结果表明，有限数量的半纯化色谱级分抑制或刺激DNA合成和细胞增殖，这种效应部分取决于级分的C或P肝脏来源。还表明，生物活性由半纯化级分中中等分子大小的肝肽介导。有人提出，肝细胞增殖的调节可能受到双重控制，通过刺激肽和抑制肽的相互作用来实现，最终结果由它们的相互作用决定。
