Levitskiĭ E L
Biokhimiia. 1982 Oct;47(10):1601-7.
The incorporation of single-stranded DNA replication intermediates (Okazaki fragments) into double-strand polymerized DNA and the chain growth of these intermediates in adult and aged rat tissues with low and high mitotic activities were studied. These processes were found to proceed in a similar manner independently of the tissue mitotic activity. However, in tissues with a low mitotic activity of adult and aged rats the incorporation of Okazaki fragments into double-stranded DNA was decelerated as compared to those with a high mitotic activity. Since the mitotic activity and ageing were shown to have similar effects on the rate of stabilization of replicating DNA secondary structure, it may be assumed that the factors responsible for regulation of DNA replication rate are altered with ageing.
研究了成年和老年大鼠组织中，单链DNA复制中间体（冈崎片段）掺入双链聚合DNA的过程，以及这些中间体在有丝分裂活性低和高的组织中的链生长情况。发现这些过程以类似方式进行，与组织的有丝分裂活性无关。然而，在成年和老年大鼠有丝分裂活性低的组织中，与有丝分裂活性高的组织相比，冈崎片段掺入双链DNA的过程减缓。由于有丝分裂活性和衰老对复制DNA二级结构的稳定速率有相似影响，因此可以假定，负责调节DNA复制速率的因素会随衰老而改变。
