Gerasimova V V
Ukr Biokhim Zh. 1977 Jan-Feb;49(1):20-4.
The content of defects of the secondary structure of DNA native and gamma-irradiated preparations from the intestinal mucosa and liver of adult (6-8 months) and old (24-26 months) rats was studied by the kinetic formaldehyde method. The content of the native DNA secondary structure defects does not depend on the animal and tissue mitotic activity. After gamma-irradiation in vitro (60Co, 550 R/min, 3 and 6 krad) of intestinal mucosa DNA solutions (tissues with a high mitotic activity) the content of the DNA defects is higher in old animals than in young ones. The injuring effect of gamma-rays is more pronounced in experiments with intestinal mucosa DNA than with liver DNA (tissue with a low mitotic activity). In liver DNA the content of the defects caused by gamma-rays does not depend on the animal age.
采用动力学甲醛法研究了成年（6 - 8个月）和老年（24 - 26个月）大鼠肠黏膜和肝脏的天然及γ射线辐照制剂中DNA二级结构的缺陷含量。天然DNA二级结构缺陷的含量不依赖于动物和组织的有丝分裂活性。在体外对肠黏膜DNA溶液（有丝分裂活性高的组织）进行γ射线辐照（60Co，550 R/分钟，3和6千拉德）后，老年动物DNA缺陷的含量高于幼年动物。γ射线对肠黏膜DNA的损伤作用在实验中比肝脏DNA（有丝分裂活性低的组织）更明显。在肝脏DNA中，γ射线引起的缺陷含量不依赖于动物年龄。
