Saitoh H
Nihon Seikeigeka Gakkai Zasshi. 1982 Jun;56(6):487-99.
Changes in muscle blood flow, PO2 and pH during and after contraction were studied on the quadriceps muscle of the anesthetized rabbits. Muscle blood flow was measured with a heated thermocouple technique, PO2 with an oxygen electrode and pH with a micro glass electrode. The muscle contraction was produced by supramaximal stimuli (7-9 V, 0.1 msec) of the femoral nerve. During weak contraction by lower frequent stimuli (1-17 Hz), muscle blood flow and muscle PO2 increased linearly in response to various frequencies of the stimulation. During strong contraction by higher frequent stimuli (more than 25 Hz), however, muscle blood flow and PO2 decreased gradually. These findings suggest the occurrence of anoxia in the muscle tissue during strong contraction. On the other hand, changes in muscle blood flow after contraction were different from those in muscle PO2. In the ischemic muscle caused by the femoral artery occlusion, increased muscle PO2 remained low level during contraction in various frequencies of the stimulation and durations of the contraction. During strong contraction of the normal and ischemic muscles, increased muscle PO2 remained low in various durations of the contraction; this seems to imply an anoxia in the muscle tissue. Muscle pH began to decrease during contraction and continued decreasing after contraction. These changes in muscle pH were correlated to the intensity and duration of the contraction, but were not similar to the changes in muscle blood flow. Changes in muscle pH after the femoral artery occlusion were different from those of muscle blood flow and from the pattern of reactive hyperemia. These findings suggest that postcontraction hyperemia and reactive hyperemia are not only due to anoxia nor acidosis in the muscle tissue, but also due to some other factors associated with the changes in muscle PO2 and pH.
在麻醉兔的股四头肌上研究了收缩期间及收缩后肌肉血流、氧分压（PO₂）和pH值的变化。用热偶技术测量肌肉血流，用氧电极测量PO₂，用微型玻璃电极测量pH值。通过股神经的超强刺激（7 - 9V，0.1毫秒）引起肌肉收缩。在较低频率刺激（1 - 17Hz）引起的弱收缩期间，肌肉血流和肌肉PO₂随刺激频率的增加呈线性增加。然而，在较高频率刺激（超过25Hz）引起的强收缩期间，肌肉血流和PO₂逐渐下降。这些发现提示在强收缩期间肌肉组织中发生了缺氧。另一方面，收缩后肌肉血流的变化与肌肉PO₂的变化不同。在股动脉闭塞引起的缺血肌肉中，在不同刺激频率和收缩持续时间的收缩过程中，升高的肌肉PO₂保持在低水平。在正常和缺血肌肉的强收缩期间，在不同收缩持续时间内升高的肌肉PO₂保持在低水平；这似乎意味着肌肉组织中存在缺氧。肌肉pH值在收缩期间开始下降，并在收缩后持续下降。这些肌肉pH值的变化与收缩的强度和持续时间相关，但与肌肉血流的变化不同。股动脉闭塞后肌肉pH值的变化与肌肉血流的变化以及反应性充血的模式不同。这些发现提示收缩后充血和反应性充血不仅是由于肌肉组织中的缺氧或酸中毒，还由于与肌肉PO₂和pH值变化相关的一些其他因素。
