由转化的大鼠细胞系产生的大基因组大小莫洛尼鼠肉瘤病毒的特性分析。
Characterization of a large genomic size Moloney murine sarcoma virus produced by a transformed rat cell line.
作者信息
Devaux J, Buendia M A, Hamelin R, Bernard C, Tavitian A
出版信息
J Gen Virol. 1982 Nov;63 (Pt 1):223-6. doi: 10.1099/0022-1317-63-1-223.
A rat cell line (78A1) transformed by the Moloney murine sarcoma virus-Moloney murine leukaemia virus (Mo-MuSV-MuLV) complex was found to produce a sarcoma virus different from the isolates previously described. Analysis of intracellular RNA of the 78A1 cell line by electrophoresis on agarose gel, and hybridization with DNA probes specific to M1-Mo-MuSV and v-mosMo sequences revealed a size of 6.7 kilobases (kb) for the RNA of this sarcoma virus. This genome is larger than those of m1, m3, HT1 and 124 isolates and slightly smaller than the myeloproliferative sarcoma virus genome (7.0 kb).
发现由莫洛尼鼠肉瘤病毒 - 莫洛尼鼠白血病病毒（Mo - MuSV - MuLV）复合物转化的大鼠细胞系（78A1）产生了一种不同于先前描述的分离株的肉瘤病毒。通过琼脂糖凝胶电泳分析78A1细胞系的细胞内RNA，并与M1 - Mo - MuSV和v - mosMo序列特异性的DNA探针杂交，发现这种肉瘤病毒的RNA大小为6.7千碱基（kb）。该基因组比m1、m3、HT1和124分离株的基因组大，比骨髓增殖性肉瘤病毒基因组（7.0 kb）略小。
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