Dekaban G A, Ball J K, Loosmore S M, McCarter J A
J Gen Virol. 1980 Apr;47(2):407-22. doi: 10.1099/0022-1317-47-2-407.
The genetic information of MoMuSV-349 and MoMuSV-124, two clones of productively transformed TB cells, was distributed between two size classes of RNA (mol. wt. 2.9 x 10(6)) in the proportions of 5:1. Some preparations of MoMuSV-124 lacked the large RNA. The virions produced by both clones also contained all the nucleotide sequences of Moloney leukaemia virus and the ratio of MuSV:MuLV produced by the two clones differed markedly. The distribution of the sequences specific for Moloney murine leukaemia virus (MoMuLV) between the two size classes of RNA was studied using molecular hybridization to DNA probes complementary to and representative of: (i) the Moloney murine sarcoma virus (MoMuSV) RNA genome (mol. wt. 1.9 x 10(6)); (ii) those nucleotide sequences shared by MoMuSV and MoMuLV; (iii) nucleotide sequences specific for MoMuSV; (iv) nucleotide sequences specific for MoMuLV. The only detectable Moloney leukaemia virus-specific nucleotide sequences present in MoMuSV-124 virions were in the RNA of mol. wt. 1.9 x 10(6), whereas these sequences were detected in the RNA of mol. wt. 2.9 x 10(6) isolated from MoMuSV-349 virions. The biological properties of the replicating information in MoMuSV-124 suggest that, consistent with the small size of RNA, it is defective. whereas MoMuSV-349 produces virions containing an intact MoMuLV genome, competent for replication.
高效转化的结核细胞的两个克隆MoMuSV - 349和MoMuSV - 124的遗传信息分布在两种大小类别的RNA（分子量2.9×10⁶）中，比例为5:1。一些MoMuSV - 124制剂缺乏大的RNA。这两个克隆产生的病毒粒子也包含莫洛尼白血病病毒的所有核苷酸序列，并且两个克隆产生的MuSV:MuLV比例明显不同。使用与以下互补并代表其的DNA探针进行分子杂交，研究了莫洛尼鼠白血病病毒（MoMuLV）特异性序列在两种大小类别的RNA之间的分布：（i）莫洛尼鼠肉瘤病毒（MoMuSV）RNA基因组（分子量1.9×10⁶）；（ii）MoMuSV和MoMuLV共有的那些核苷酸序列；（iii）MoMuSV特异性核苷酸序列；（iv）MoMuLV特异性核苷酸序列。在MoMuSV - 124病毒粒子中唯一可检测到的莫洛尼白血病病毒特异性核苷酸序列存在于分子量1.9×10⁶的RNA中，而这些序列在从MoMuSV - 349病毒粒子中分离出的分子量2.9×10⁶的RNA中被检测到。MoMuSV - 124中复制信息的生物学特性表明，与RNA的小尺寸一致，它是有缺陷的。而MoMuSV - 349产生含有完整MoMuLV基因组、能够复制的病毒粒子。
