Issel E P, Lun A, Pohle R, Gross J
J Perinat Med. 1982;10(5):221-5. doi: 10.1515/jpme.1982.10.5.221.
During hypoxia there is an increased formation of hypoxanthine from the consumption of ATP; simultaneously the oxidation into uric acid is decreased. The purpose of this study was to determine possible correlations between hypoxanthine concentrations in the amniotic fluid and states of fetal hypoxia. We obtained 83 amniotic fluid samples from 68 patients during pregnancy or delivery. Hypoxanthine was assayed fluorimetrically according to GARDINER [4]. In the course of pregnancy, hypoxanthine levels in the amniotic fluid rise slightly. A marked increase occurs during delivery (without labor mean = 3.4 +/- 0.9 mumol/l, with labor mean = 7.0 +/- 5.4 mumol/l, p less than 0.02). There is no significant difference in the levels obtained from the first and second stages of labor. In intrauterine fetal death (Fig. 1) and in depressed newborns (Tab. I) there were increased hypoxanthine levels in the amniotic fluid, this was not seen in other complications of pregnancy. Independently from the fetal state increased hypoxanthine concentrations may occur in the amniotic fluid evidently as a consequence of labor activity. Thus the determination of hypoxanthine levels in the amniotic fluid cannot be used for the diagnosis of fetal hypoxia.
在缺氧状态下，ATP消耗会导致次黄嘌呤生成增加；同时，其氧化为尿酸的过程减少。本研究的目的是确定羊水次黄嘌呤浓度与胎儿缺氧状态之间可能存在的相关性。我们在妊娠期间或分娩时从68例患者中获取了83份羊水样本。根据加德纳[4]的方法，采用荧光法测定次黄嘌呤。在妊娠过程中，羊水次黄嘌呤水平略有上升。分娩期间会出现显著升高（未临产时平均值 = 3.4±0.9μmol/L，临产时平均值 = 7.0±5.4μmol/L，p<0.02）。第一产程和第二产程获得的水平无显著差异。在宫内胎儿死亡（图1）和新生儿窒息（表I）时，羊水次黄嘌呤水平升高，而在妊娠的其他并发症中未观察到这种情况。显然，由于产程活动，无论胎儿状态如何，羊水中次黄嘌呤浓度都可能升高。因此，测定羊水中次黄嘌呤水平不能用于诊断胎儿缺氧。
