Soltis R D, Hasz D
J Clin Lab Immunol. 1982 Oct;9(1):13-7.
We have previously demonstrated that isolated IgG, when added to buffer or serum, undergoes aggregation during incubation at 37 degrees C. The addition of albumin inhibits aggregation. The present studies were designed to determine whether endogenous immunoglobulins in normal serum, present in low-normal concentration, would likewise aggregate in the presence of hypoalbuminemia. Albumin was removed from normal serum by absorption with Affi-gel Blue. Following incubation at 37 degrees C, high molecular weight IgG, IgM and IgA were detectable by density gradient ultracentrifugation. Reconstituting the samples with albumin prior to incubation prevented the formation of immunoglobulin aggregates. These data suggest that immunoglobulin aggregation might also occur in vivo in a variety of pathological conditions associated with hypoalbuminemia and/or hypergammaglobulinemia and might be incorrectly assumed to represent antigen-antibody complexes.
我们之前已经证明，分离出的免疫球蛋白G（IgG），当添加到缓冲液或血清中时，在37℃孵育期间会发生聚集。添加白蛋白可抑制聚集。本研究旨在确定正常血清中浓度处于低正常水平的内源性免疫球蛋白在低白蛋白血症情况下是否同样会聚集。通过用Affi -凝胶蓝吸附从正常血清中去除白蛋白。在37℃孵育后，通过密度梯度超速离心可检测到高分子量的IgG、IgM和IgA。在孵育前用白蛋白重构样品可防止免疫球蛋白聚集体的形成。这些数据表明，免疫球蛋白聚集也可能在体内与低白蛋白血症和/或高球蛋白血症相关的多种病理状况下发生，并且可能被错误地认为代表抗原 - 抗体复合物。
