Hebb J H, Crooks C R, Caplan Y H, Mergner W J
J Anal Toxicol. 1982 Sep-Oct;6(5):206-8. doi: 10.1093/jat/6.5.206.
A sensitive and reliable procedure is presented for the measurement of the common tricyclic antidepressant (TCA) drugs in a variety of biological specimens at therapeutic as well as toxic concentrations. The method employs GC/NPD and a column packed with 3% OV-17 on Chromosorb W-HP. The pH of specimen extraction was optimized at 8.6, and 1-chlorobutane:ethyl ether (3:1) was found to be an efficient solvent for the primary extraction. Recoveries for all TCA drugs were at least 50% for each specimen. Linearity of extraction was observed over a range of therapeutic and toxic concentrations with a correlation coefficient greater than 0.99.
本文介绍了一种灵敏可靠的方法，用于测量各种生物样本中治疗浓度和中毒浓度的常见三环类抗抑郁药（TCA）。该方法采用气相色谱/氮磷检测器（GC/NPD）以及填充有3% OV-17的Chromosorb W-HP柱。将样本提取的pH值优化为8.6，发现1-氯丁烷：乙醚（3:1）是初次提取的有效溶剂。每个样本中所有TCA药物的回收率至少为50%。在一系列治疗浓度和中毒浓度范围内观察到提取的线性，相关系数大于0.99。
