Crémieux A, Guiraud H, Duménil G, Chevalier J
Ann Microbiol (Paris). 1978 Jul;129B(1):49-56.
This work describes a technique of emulsification of mycobacterial cells (including tubercle bacilli) in order to obtain homogeneous suspensions containing up to 10(8) cells/ml. The suspensions are used in a modified AFNOR method for the evaluation of bacterial activity: within five min virulent strains are more resistant than are avirulent ones but, within 30 min and in the presence of proteins, the behaviour of all strains is quite similar.
