Descombes P, Olivier J P, Vischer T L
Agents Actions. 1982 Oct;12(4):499-502. doi: 10.1007/BF01965933.
The effect of protease inhibitors on the immune response in mice was investigated. Soybean inhibitor, aprotinin, alpha 1-antitrypsin and ovomucoid (1 mg) diminished significantly the direct plaque forming cell response per spleen 3 days after immunization with 10(8) sheep erythrocytes (SRBC), when given at the same time as the antigen. With alpha 1-antitrypsin, the effect was not significant 4 days after immunization. With soybean inhibitor, no significant suppressive effect was seen, when the inhibitor was given either 24 hours before or after the antigen. Both aprotinin and ovomucoid diminished a secondary response to SRBC, limited to the indirect plaque forming cells. Soybean inhibitor had an equivocal effect on the secondary response. A suppressive effect of soybean inhibitor, but not ovomucoid was seen using KLH-FITC or LPS-FITC as antigens. Neither soybean inhibitor nor aprotinin had an effect on the induction of delayed-type skin reactivity to oxazolone or FITC.
研究了蛋白酶抑制剂对小鼠免疫反应的影响。大豆抑制剂、抑肽酶、α1抗胰蛋白酶和卵类粘蛋白（1毫克）在与10⁸个绵羊红细胞（SRBC）同时给予作为抗原时，免疫后3天显著降低了每脾脏的直接空斑形成细胞反应。对于α1抗胰蛋白酶，免疫后4天效果不显著。对于大豆抑制剂，在抗原前24小时或后给予时，未观察到显著的抑制作用。抑肽酶和卵类粘蛋白均降低了对SRBC的二次反应，仅限于间接空斑形成细胞。大豆抑制剂对二次反应的影响不明确。以钥孔戚血蓝蛋白-异硫氰酸荧光素（KLH-FITC）或脂多糖-异硫氰酸荧光素（LPS-FITC）作为抗原时，观察到大豆抑制剂有抑制作用，但卵类粘蛋白没有。大豆抑制剂和抑肽酶对恶唑酮或异硫氰酸荧光素迟发型皮肤反应的诱导均无影响。
