Strum J M
Anat Rec. 1982 Dec;204(4):323-32. doi: 10.1002/ar.1092040405.
The purpose of this study was to determine by use of light- and electron-microscope autoradiography whether or not iodination occurred in mammary tumors of female GR mice. Of the sixty tumors studied it was found that pregnancy-dependent and hormone-induced tumors possessed iodinating ability. Although mammary glands from nonpregnant GR mice lacked the ability to iodinate, by the 16th day of pregnancy in response to hormonal stimulation the glands readily iodinated casein, and some epithelial cells contained ultrastructural cytochemical evidence of mammary peroxidase. Preneoplastic mammary gland lesions known as hyperplastic alveolar nodules were also able to iodinate, as were plaques, the disc-shaped lesions which give rise to the hormone-responsive mammary tumors in this strain. Plaques also contained epithelial cells with mammary peroxidase activity. When hormone-induced mammary tumors were transplanted into syngeneic mice they retained the ability to iodinate for several generations. However, as the tumors progressed to hormone independence, the ability to iodinate was gradually lost. Hormone-independent mammary tumors from GR mice lacked both iodinating ability and cytochemical evidence of mammary peroxidase. These findings suggest that iodination depends upon hormone-responsive cells within the mammary tumors and that as these cells become hormone unresponsive, the ability to iodinate is lost.
本研究的目的是通过光学显微镜和电子显微镜放射自显影术来确定雌性GR小鼠乳腺肿瘤中是否发生碘化作用。在所研究的60个肿瘤中，发现依赖妊娠和激素诱导的肿瘤具有碘化能力。虽然未怀孕的GR小鼠的乳腺缺乏碘化能力，但在妊娠第16天时，由于激素刺激，乳腺很容易对酪蛋白进行碘化，并且一些上皮细胞含有乳腺过氧化物酶的超微结构细胞化学证据。称为增生性肺泡结节的癌前乳腺病变也能够碘化，斑块也是如此，这种斑块是在该品系中产生激素反应性乳腺肿瘤的盘状病变。斑块中也含有具有乳腺过氧化物酶活性的上皮细胞。当激素诱导的乳腺肿瘤移植到同基因小鼠中时，它们在几代内都保留了碘化能力。然而，随着肿瘤发展为激素非依赖性，碘化能力逐渐丧失。来自GR小鼠的激素非依赖性乳腺肿瘤既缺乏碘化能力，也缺乏乳腺过氧化物酶的细胞化学证据。这些发现表明，碘化作用取决于乳腺肿瘤内对激素有反应的细胞，并且随着这些细胞对激素变得无反应，碘化能力就会丧失。
