Golovkina T V, Prakash O, Ross S R
Department of Microbiology/Cancer Center, University of Pennsylvania, School of Medicine, Philadelphia, 19104-6142, USA.
Virology. 1996 Apr 1;218(1):14-22. doi: 10.1006/viro.1996.0161.
Mtv-2 is an endogenous mouse mammary tumor virus (MMTV) that is responsible for the induction of mammary gland tumors in the high mammary gland tumor-incidence strain GR. GR animals inherit four different endogenous MMTVs in addition to Mtv-2: Mtv-3, Mtv-7, Mtv-8 and Mtv-17. In this study we analyzed the involvement of these nonpathogenic endogenous proviruses in the mammary gland tumors caused by Mtv-2. We showed that Mtv-17 is expressed in the mammary gland of GR mice, efficiently packaged into virions, and shed into milk. DNA isolated from both mammary gland tumors and the nonmalignant mammary gland tissues of GR mice contained amplified copies of both newly acquired Mtv-2 and recombinant proviruses with the env gene derived from Mtv-17. A small percentage of these tumors contained predominantly recombinant viruses. These findings suggest a role for Mtv-17 in the mammary gland tumors induced by Mtv-2 in GR mice.
Mtv-2是一种内源性小鼠乳腺肿瘤病毒（MMTV），它可在高乳腺肿瘤发病率的GR品系小鼠中诱发乳腺肿瘤。除Mtv-2外，GR品系动物还继承了四种不同的内源性MMTV：Mtv-3、Mtv-7、Mtv-8和Mtv-17。在本研究中，我们分析了这些非致病性内源性前病毒在由Mtv-2引起的乳腺肿瘤中的作用。我们发现Mtv-17在GR小鼠的乳腺中表达，能有效地包装进病毒粒子，并分泌到乳汁中。从GR小鼠的乳腺肿瘤和非恶性乳腺组织中分离出的DNA，均含有新获得的Mtv-2以及带有源自Mtv-17的env基因的重组前病毒的扩增拷贝。这些肿瘤中有一小部分主要含有重组病毒。这些发现表明Mtv-17在GR小鼠中由Mtv-2诱发的乳腺肿瘤中发挥了作用。
