Kim H, Oden E, Lin C
Antimicrob Agents Chemother. 1982 Nov;22(5):848-51. doi: 10.1128/AAC.22.5.848.
A high-pressure liquid chromatographic method was developed for quantitative determination in human serum of a new penem antibiotic known as Sch 29482 (5R,6S,8R-2-ethylthio-6(1-hydroxyethyl)penem-3-carboxylic acid). The method involves serum extraction at an acid pH with ether, followed by separation on a reverse-phase column and UV light detection at 254 nm. This technique produced a good linear relationship between the peak height ratio and the Sch 29482 concentration, which ranged from 0.09 to 35.64 micrograms/ml. In addition, this procedure proved to be quite specific for Sch 29482, since all beta-lactam antibiotics tested did not interfere with the assay. For high concentrations (greater than 0.5 micrograms/ml), the mean values obtained from the high-pressure liquid chromatographic method correlated very well (r = 0.997) with those obtained from a microbiological assay. This method is accurate and reproducible, with a sensitivity of about 0.09 micrograms per ml of serum. It is useful for monitoring serum drug levels in animals and humans and can also be used for drug pharmacokinetic studies in humans.
已开发出一种高压液相色谱法，用于定量测定人血清中一种名为Sch 29482（5R，6S，8R-2-乙硫基-6（1-羟乙基）青霉烯-3-羧酸）的新型青霉烯抗生素。该方法包括在酸性pH下用乙醚萃取血清，然后在反相柱上分离，并在254nm处进行紫外光检测。该技术在峰高比与Sch 29482浓度（范围为0.09至35.64微克/毫升）之间产生了良好的线性关系。此外，该方法对Sch 29482具有很高的特异性，因为所有测试的β-内酰胺抗生素均不干扰测定。对于高浓度（大于0.5微克/毫升），高压液相色谱法获得的平均值与微生物测定法获得的平均值相关性非常好（r = 0.997）。该方法准确且可重复，血清灵敏度约为0.09微克/毫升。它可用于监测动物和人类的血清药物水平，也可用于人类药物药代动力学研究。
