Iakovlev V A, Levchuk T P, Malakhova E A
Vopr Med Khim. 1976 May-Jun;22(3):417-21.
A method is developed for determination of the lipoprotein lipase activity. The method is based on the steady state potentiometric titration at constant pH value of higher fatty acids, liberated during the hydrolysis. An oil emulsion intralipid, activated by human blood serum, was used as a substrate. The sensitivity of the method was equal to 0.004-0.010 micronM of fatty acids per min. The reproducibility of the results was 2-5%. This simple and rapid method enabled to study kinetic of reactions, catalyzed by lipoprotein lipases.
开发了一种测定脂蛋白脂肪酶活性的方法。该方法基于在水解过程中释放的高级脂肪酸在恒定pH值下的稳态电位滴定。使用经人血清激活的油乳剂英脱利匹特作为底物。该方法的灵敏度为每分钟0.004 - 0.010微摩尔脂肪酸。结果的重现性为2 - 5%。这种简单快速的方法能够研究由脂蛋白脂肪酶催化的反应动力学。
