Kalovidouris A E, Johnson R L
Ann Rheum Dis. 1978 Oct;37(5):444-8. doi: 10.1136/ard.37.5.444.
A rapid screening method for serum cryoblobulin is reported. It requires only common laboratory equipment and is based upon the detection of light scattering (500 millimicron) in the early phase of cryoaggregation. All of 28 sera negative for cryoglobulins by the conventional 5-day incubation method were negative by the screening method. Conversely, all sera containing 60 microgram/ml or more of mixed cryoglobulins were positive by the screening method. The initiation phase of cryoprecipitation in mixed cryoglobulins was found to be prompt, as reported previously for monoclonal cryoglobulins. This sensitive method of cryoglobulin detection provides results to the clinician within 2 hours, a helpful insight where consideration of possible immune complex vasculitis exists.
本文报道了一种血清冷球蛋白快速筛查方法。该方法仅需常规实验室设备，基于在冷凝聚早期对光散射（500毫微米）的检测。采用传统的5天孵育法检测为冷球蛋白阴性的28份血清，用该筛查方法检测均为阴性。相反，所有含有60微克/毫升或更多混合冷球蛋白的血清，用该筛查方法检测均为阳性。如先前关于单克隆冷球蛋白的报道，发现混合冷球蛋白的冷沉淀起始阶段迅速。这种敏感的冷球蛋白检测方法可在2小时内向临床医生提供结果，有助于了解可能存在免疫复合物性血管炎的情况。
