根瘤菌的类胡萝卜素。II. 尼古丁对羽扇豆根瘤菌类胡萝卜素模式的影响。

Carotenoids of rhizobia. II. The effect of nicotine on the carotenoid pattern of Rhizobium lupini.

作者信息

Kleinig H, Meister W, Englert G

出版信息

Arch Microbiol. 1978 Oct 4;119(1):71-4. doi: 10.1007/BF00407930.

Abstract

With increasing concentrations in the growth medium of the cyclization inhibitors nicotine or 2-(4-chlorophenylthio)-triethylamine hydrochloride (CPTA) the previously identified bicyclic carotenoids of Rhizobium lupini (2,3,2',3'-tetrahydroxy-beta,beta-caroten-4-one and 2,3,2',3'-tetrahydroxy-beta,beta-carotene) were successively replaced by hitherto unknown monocyclic carotenoids. By application of mass and nuclear magnetic resonance spectroscopy 3 carotenoids were identified as 2,3-trans-dihydroxy-beta,psi-caroten-4-one, 2,3-trans-dihydroxy-beta,psi-carotene, and 3-hydroxy-beta,psi-caroten-4-one. A further compound was tentatively established as (2- or 3-)monohydroxy-beta,psi-carotene. It was found that other inhibitors such as diphenylamine or 4-chloro-5-(dimethylamino)-2-alpha,alpha,alpha(trifluoro-m-tolyl)-3-(2H)-pyridazinone (San 6706) did not affect the pigment pattern. The results are discussed in relation to carotenoid biosynthesis in Rhizobium lupini.

摘要

随着环化抑制剂尼古丁或2-(4-氯苯硫基)-三乙胺盐酸盐(CPTA)在生长培养基中的浓度增加，先前鉴定出的羽扇豆根瘤菌的双环类胡萝卜素(2,3,2',3'-四羟基-β,β-胡萝卜素-4-酮和2,3,2',3'-四羟基-β,β-胡萝卜素)逐渐被迄今未知的单环类胡萝卜素所取代。通过质谱和核磁共振光谱分析，鉴定出3种类胡萝卜素为2,3-反式-二羟基-β,ψ-胡萝卜素-4-酮、2,3-反式-二羟基-β,ψ-胡萝卜素和3-羟基-β,ψ-胡萝卜素-4-酮。另一种化合物初步确定为(2-或3-)单羟基-β,ψ-胡萝卜素。研究发现，其他抑制剂如二苯胺或4-氯-5-(二甲基氨基)-2-α,α,α-(三氟间甲苯基)-3-(2H)-哒嗪酮(San 6706)不会影响色素模式。结合羽扇豆根瘤菌中类胡萝卜素的生物合成对结果进行了讨论。