Leutwiler L S, Chapman D J
Arch Microbiol. 1979;123(3):267-73. doi: 10.1007/BF00406660.
The biosynthesis of the aryl carotenoid, chlorobactene, was examined in the green sulfur bacterium, Chlorobium limicola f. thiosulfatophilum. Nicotine, which was used to inhibit carotenoid cyclization, caused the accumulation of the acyclic carotenoid, lycopene. Cells reincubated in fresh medium, after removal of nicotine, synthesized chlorobactene more readily from newly synthesized lycopene rather than from the pool of lycopene accumulated during nicotine inhibition. When the cells were reincubated in the presence of diphenylamine, which inhibited de novo carotenogenesis, a portion of the lycopene which had accumulated during nicotine inhibition was converted into chlorobactene. There was no evidence that neurosporene, rather than lycopene, was the precyclization intermediate. The involvement of gamma-carotene as the cyclic precursor of chlorobactene also was shown. The pathway for chlorobactene biosynthesis is discussed in terms of a possible arrangement of the enzymes involved in carotenoid biosynthesis.
在绿色硫细菌嗜硫代硫酸盐绿胶菌（Chlorobium limicola f. thiosulfatophilum）中研究了芳基类胡萝卜素氯菌烯的生物合成。用于抑制类胡萝卜素环化的尼古丁导致无环类胡萝卜素番茄红素的积累。去除尼古丁后，在新鲜培养基中重新培养的细胞，从新合成的番茄红素而不是从尼古丁抑制期间积累的番茄红素池中更容易合成氯菌烯。当细胞在抑制从头类胡萝卜素生成的二苯胺存在下重新培养时，尼古丁抑制期间积累的一部分番茄红素被转化为氯菌烯。没有证据表明神经孢烯而非番茄红素是环化前体中间体。还表明了γ-胡萝卜素作为氯菌烯的环状前体的参与情况。根据类胡萝卜素生物合成中涉及的酶的可能排列讨论了氯菌烯生物合成的途径。
