在小规模下通过固相磷三酯法合成寡脱氧核糖核苷酸；制备用于改进启动子序列的寡核苷酸。

Synthesis of oligodeoxyribonucleotides by solid phase phosphotriester method on a reduced scale; preparation of oligonucleotides for improved promoter sequence.

作者信息

Naruto M

出版信息

Nucleic Acids Symp Ser. 1982(11):77-80.

Abstract

A small reaction vessel, composed of a balled sintered glass filter and a test tube with a stopcock, was designed for the solid phase phosphotriester synthesis of oligodeoxyribonucleotide. Operation of small scale synthesis (nucleoside on resin: 3-6 mumol, activated diester of dimer: 20-30 mumol) was performed under the atmosphere of argon. The yield of each coupling reaction was 60-100%. Twelve short oligonucleotides (6-16mer) were obtained in 19-78% overall yield by this method. These oligomers were prepared as a part of the control region of gene to increase the translation efficiency.

摘要

设计了一种由球形烧结玻璃过滤器和带旋塞的试管组成的小型反应容器，用于寡脱氧核糖核苷酸的固相磷三酯合成。小规模合成（树脂上的核苷：3 - 6 μmol，二聚体的活化二酯：20 - 30 μmol）在氩气气氛下进行。每个偶联反应的产率为60 - 100%。通过这种方法以19 - 78%的总产率获得了12条短寡核苷酸（6 - 16聚体）。这些寡聚物作为基因控制区的一部分来制备，以提高翻译效率。

相似文献

1

Synthesis of oligodeoxyribonucleotides by solid phase phosphotriester method on a reduced scale; preparation of oligonucleotides for improved promoter sequence.在小规模下通过固相磷三酯法合成寡脱氧核糖核苷酸；制备用于改进启动子序列的寡核苷酸。

Nucleic Acids Symp Ser. 1982(11):77-80.

2

Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡聚脱氧核糖核苷酸的快速合成。V. 通过磷酸三酯中间体进行寡聚脱氧核糖核苷酸固相合成的进一步研究。

Nucleic Acids Symp Ser. 1980(7):243-57.

3

Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method.多核苷酸的固相合成：V. 用磷酸单三唑酯法合成寡脱氧核糖核苷酸

Nucleic Acids Symp Ser. 1980(7):281-91.

4

Programmed flow preparation of DNA-oligomers.DNA 寡聚物的程序化流动制备

Nucleic Acids Symp Ser. 1982(11):85-8.

5

Synthesis of oligonucleotides containing the hypermodified base, alpha-putrescinylthymine.含超修饰碱基α-腐胺基胸腺嘧啶的寡核苷酸的合成

Nucleic Acids Symp Ser. 1984(15):101-4.

6

Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。

Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.

7

A novel class of condensing reagents in phosphodiester oligodeoxyribonucleotides synthesis. Application of the constituents of free terminal carboxy oxytocine gene.磷酸二酯寡脱氧核糖核苷酸合成中的一类新型缩合试剂。游离末端羧基催产素基因成分的应用。

Nucleic Acids Symp Ser. 1980(7):23-37.

8

Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.在交联聚丙烯酰吗啉载体上进行寡核苷酸的固相合成。

Nucleic Acids Symp Ser. 1980(7):233-41.

9

Solvent extraction method used in separating the protected product of synthetic oligodeoxyribonucleotides.

Nucleic Acids Symp Ser. 1980(7):89-98.

10

Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).寡核苷酸在纤维素上的合成。第二部分：编码胃抑制多肽（GIP）的22个寡脱氧核苷酸的合成设计与合成策略。

Nucleic Acids Symp Ser. 1980(7):225-32.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。