Téoule R, Derbyshire R, Guy A, Molko D, Roget A
Nucleic Acids Symp Ser. 1980(7):23-37.
Benzotriazol-1-yl-oxy-tris (dimethylamino) phosphonium salts have been used as novel condensing agents to promote internucleotide bond formation in phosphotriester oligodeoxyribonucleotide synthesis. The effectiveness of these stable compounds has been shown by the synthesis of the six oligodeoxyribonucleotide building blocks of the carboxy terminal oxytocine gene. Some modifications incorporated in the phosphotriester method include a rapid procedure to prepare the fully protected dideoxyribonucleotide blocks and size exclusion chromatography of the deprotected oligodeoxyribonucleotides. The triester oligodeoxyribonucleotides were studied by chemical ionization mass spectrometry.
苯并三唑 -1- 基 - 氧基 - 三（二甲氨基）鏻盐已被用作新型缩合剂，以促进磷酸三酯寡脱氧核糖核苷酸合成中核苷酸间键的形成。羧基末端催产素基因的六个寡脱氧核糖核苷酸结构单元的合成已证明了这些稳定化合物的有效性。磷酸三酯法中引入的一些改进包括制备完全保护的双脱氧核糖核苷酸结构单元的快速程序以及脱保护寡脱氧核糖核苷酸的尺寸排阻色谱法。通过化学电离质谱法对三酯寡脱氧核糖核苷酸进行了研究。
