Shinomiya T, Sawai H
Nucleic Acids Symp Ser. 1982(11):93-6.
Antibody to 2'-5' linked triadenylic acid, A2'p5'A2'p5'A was prepared and purified by affinity chromatography. [8-14C]-A2'p5'A2'p5'A used in immunochemical studies was synthesized by nonenzymatic template-directed condensation. The specificity of the antibody was determined by the inhibition of the binding of the radioisotope probe to the antibody by a competitor. The cross reactivity of a number of modified oligonucleotides has been evaluated in order to gain information on the structural feature involved in the binding to the antibody. The adenine moiety and 2'-5' internucleotide linkage are crucial region for the binding. The cross reactivity of the modified oligonucleotides is indicated to have relation to the biological activity of the corresponding 5'-phosphorylated oligonucleotides.
制备了针对2'-5'连接的三腺苷酸（A2'p5'A2'p5'A）的抗体，并通过亲和层析进行纯化。免疫化学研究中使用的[8-14C]-A2'p5'A2'p5'A是通过非酶模板导向缩合合成的。抗体的特异性通过竞争者对放射性同位素探针与抗体结合的抑制来确定。为了获得有关与抗体结合所涉及的结构特征的信息，评估了多种修饰寡核苷酸的交叉反应性。腺嘌呤部分和2'-5'核苷酸间连接是结合的关键区域。结果表明，修饰寡核苷酸的交叉反应性与相应5'-磷酸化寡核苷酸的生物活性有关。
