Laurence L, Marti J, Roux D, Cailla H
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2322-6. doi: 10.1073/pnas.81.8.2322.
A monoclonal antibody highly specific for (2'-5')adenylyladenosine oligonucleotides was used together with a 125I-labeled analog of this compound to detect and quantify phosphorylated and nonphosphorylated (2'-5')adenylyladenosine oligonucleotides in a variety of tissues and cells. These oligonucleotides were first assayed as a whole in perchloric acid extracts and then further individually characterized by HPLC analysis. Their sensitivity to alkaline phosphatase, snake venom phosphodiesterase, and T2 RNase was systematically checked. Nonphosphorylated (2'-5')adenylyladenosine oligonucleotides were found in mammalian tissues as well as in yeast and bacteria. In normal mouse brain, lung, heart, pancreas, spleen, kidney, and liver their concentrations ranged from 10 to 200 pmol/g wet weight, depending on tissue and strain. The oligonucleotides were mainly dimers, trimers, tetramers, and pentamers. In addition, phosphorylated (2'-5')adenylyladenosine oligonucleotides were shown in liver and kidney extracts.
一种对（2'-5'）腺苷酸寡核苷酸具有高度特异性的单克隆抗体，与该化合物的125I标记类似物一起，用于检测和定量多种组织和细胞中磷酸化和非磷酸化的（2'-5'）腺苷酸寡核苷酸。这些寡核苷酸首先在高氯酸提取物中作为一个整体进行检测，然后通过高效液相色谱分析进一步单独表征。系统地检查了它们对碱性磷酸酶、蛇毒磷酸二酯酶和T2核糖核酸酶的敏感性。在哺乳动物组织以及酵母和细菌中均发现了非磷酸化的（2'-5'）腺苷酸寡核苷酸。在正常小鼠的脑、肺、心脏、胰腺、脾脏、肾脏和肝脏中，其浓度范围为每克湿重10至200皮摩尔，具体取决于组织和品系。这些寡核苷酸主要是二聚体、三聚体、四聚体和五聚体。此外，在肝脏和肾脏提取物中显示出磷酸化的（2'-5'）腺苷酸寡核苷酸。
