Ichihashi H, Kondo T
Gan To Kagaku Ryoho. 1982 Apr;9(4):575-81.
A test system using dehydrogenase activity for predicting the response to chemotherapeutic agents against cancer cells was introduced. Agar plate assay, INK which test, and SDI test commonly employed in the clinical study were also reviewed. Agar plate assay resembles antibiotic disc sensitivity test. The cancer uniformly suspended in the agar medium was exposed to drugs on paper discs for few hours. After removal of the disc, methylene blue or 2, 6-dichlorophenol indophenol was applied as a dehydrogenase indicator. INK test was introduced by Nishioka et al. in 1957. Several fragments of fresh cancer tissue were incubated with chemotherapeutic agents in roller test tubes. Twenty-four hours later, 2, 6-dichlorophenol indophenol was applied as a dehydrogenase indicator. To develop a simple, rapid, and comparable test, SDI test was introduced by us in 1964. A fresh cancer tissue was minced and made into the cell suspension. After cancer cells were exposed to chemotherapeutic agents, the activities of succinic dehydrogenase of the treated cells were measured by the reduction of 2, 3, 5-triphenyl tetrazolium chloride. Some points to be improved were investigated and discussed.
介绍了一种利用脱氢酶活性预测癌细胞对化疗药物反应的测试系统。还回顾了临床研究中常用的琼脂平板试验、INK试验和SDI试验。琼脂平板试验类似于抗生素纸片敏感性试验。将均匀悬浮在琼脂培养基中的癌细胞暴露于纸片上的药物中数小时。取出纸片后，用亚甲基蓝或2,6-二氯酚靛酚作为脱氢酶指示剂。INK试验由西冈等人于1957年提出。将几块新鲜癌组织在旋转试管中与化疗药物一起孵育。24小时后，用2,6-二氯酚靛酚作为脱氢酶指示剂。为了开发一种简单、快速且具有可比性的试验，我们于1964年引入了SDI试验。将新鲜癌组织切碎制成细胞悬液。癌细胞暴露于化疗药物后，通过2,3,5-三苯基氯化四氮唑的还原测定处理后细胞的琥珀酸脱氢酶活性。对一些需要改进的地方进行了研究和讨论。
