Holst E, Skarin A, Mårdh P A
Eur J Clin Microbiol. 1982 Oct;1(5):310-6. doi: 10.1007/BF02019978.
Comma-shaped rods isolated from the vagina of women with signs of vaginitis were studied. Three types of rods were found: a long (length about 4 microns), a medium-sized (about 3 microns) and a short (about 1 micron) variant. All three variants had a characteristic cork-screw motility. The long and the medium-sized variants had up to eight flagella, while the short had up to four. The bacteria only grew under anaerobic conditions, although after a great number of passages on artificial culture media the short variant, but not the other variants, could be grown in a microaerophilic atmosphere. In broth media growth of all three variants was stimulated by the addition of 0.3% formatefumarate. On solid media colonies developed after incubation for 48 to 72 hours at 37 degrees C. The long and the medium-sized variants differed from the short variant in that they produced leucine arylamidase, while only the short variant produced alpha-galactosidase. None of the strains utilized carbohydrates; intravariant differences were found in the test results for gelatin and nitrate reduction. All strains of the short variant tested, but none of the long, hydrolysed sodium hippurate. Gas chromatographic analyses showed that all three variants produce acetic, lactic and succinic acids.
对从有阴道炎症状的女性阴道中分离出的逗号形杆菌进行了研究。发现了三种类型的杆菌：一种长的（长度约4微米）、一种中等大小的（约3微米）和一种短的（约1微米）变体。所有这三种变体都有典型的螺旋状运动。长变体和中等大小变体有多达八条鞭毛，而短变体有多达四条。这些细菌仅在厌氧条件下生长，不过在人工培养基上多次传代后，短变体而非其他变体能够在微需氧环境中生长。在肉汤培养基中，添加0.3%的甲酸盐 - 富马酸盐可促进所有三种变体的生长。在固体培养基上，于37℃孵育48至72小时后形成菌落。长变体和中等大小变体与短变体的不同之处在于，它们产生亮氨酸芳基酰胺酶，而只有短变体产生α - 半乳糖苷酶。这些菌株均不利用碳水化合物；在明胶和硝酸盐还原试验结果中发现了变体内部的差异。所测试的所有短变体菌株，但长变体菌株均未水解马尿酸钠。气相色谱分析表明，所有三种变体都产生乙酸、乳酸和琥珀酸。
