[连续流动分析仪测定血尿素方法的综述及连续流动分析仪完全酶促紫外法测定尿素的建议]
[Review of the methods of determination of blood urea with continuous-flow analyzers and a proposal of a completely enzymatic UV method for urea by a continuous-flow analyzer].
作者信息
Sambenedetto A, Marrama P, Ottavi P F, Castronuovo A
出版信息
Quad Sclavo Diagn. 1982 Dec;18(4):440-6.
PMID:7186646
Abstract
After a brief review of the methods for determination of urea by continuous flow analyzers, a method is described based on the urease splitting of urea followed by NH3 reaction with alfa-ketoglutarate + NADH2 catalysed by GLDH. The method has been applied to continuous flow analyzers and seems to be promising.
摘要
在简要回顾了连续流动分析仪测定尿素的方法之后，本文描述了一种基于尿素经脲酶分解后，氨与α-酮戊二酸 + 还原型辅酶I在谷氨酸脱氢酶催化下反应的方法。该方法已应用于连续流动分析仪，且似乎很有前景。
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