Lustenberger P, Orsonneau J L, Cornu G, Bernard S
Ann Biol Clin (Paris). 1977;35(6):481-4.
An automated continuous flow method is described for the assay of plasmatic urea after hydrolysis by urease followed by NH+4 assay with glutamic-deshydrogenase. Precision and accuracy, comparison with chimical diacetyl-monoxim method are studied. The interaction of endogenous NH+4, the cost of the method are calculated.
描述了一种自动化连续流动法,用于在脲酶水解血浆尿素后,通过谷氨酸脱氢酶测定铵离子(NH₄⁺)。研究了该方法的精密度和准确性,并与化学双乙酰一肟法进行了比较。计算了内源性铵离子(NH₄⁺)的相互作用及该方法的成本。