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[使用酶的连续流动法分析血浆尿素]

[Analysis of plasma urea by continuous flow method using enzymes].

作者信息

Lustenberger P, Orsonneau J L, Cornu G, Bernard S

出版信息

Ann Biol Clin (Paris). 1977;35(6):481-4.

PMID:615508
Abstract

An automated continuous flow method is described for the assay of plasmatic urea after hydrolysis by urease followed by NH+4 assay with glutamic-deshydrogenase. Precision and accuracy, comparison with chimical diacetyl-monoxim method are studied. The interaction of endogenous NH+4, the cost of the method are calculated.

摘要

描述了一种自动化连续流动法,用于在脲酶水解血浆尿素后,通过谷氨酸脱氢酶测定铵离子(NH₄⁺)。研究了该方法的精密度和准确性,并与化学双乙酰一肟法进行了比较。计算了内源性铵离子(NH₄⁺)的相互作用及该方法的成本。

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