基于有丝分裂重组构建的盘基网柄菌遗传图谱。
A genetic map of Dictyostelium discoideum based on mitotic recombination.
作者信息
Welker D L, Williams K L
出版信息
Genetics. 1982 Dec;102(4):691-710. doi: 10.1093/genetics/102.4.691.
Abstract
A genetic map of the cellular slime mold Dictyostelium discoideum is presented in which 42 loci are ordered on five of the seven linkage groups. Although most of the loci were ordered using standing mitotic crossing-over techniques in which recessive selective markers were employed, use was also made of unselected recombined haploid strains. Consistent with cytological studies in which the chromosomes appear to be acrocentric, only a single arm has been found for each of the five linkage groups studied. The mating-type locus, matA, has been located in the tsgE-sprA interval on linkage group I on the basis of studies on diploids formed between strains of opposite mating type that have escaped from vegetative incompatibility.
摘要
本文展示了盘基网柄菌的细胞遗传图谱，其中42个基因座排列在七个连锁群中的五个上。尽管大多数基因座是通过使用隐性选择标记的常规有丝分裂交换技术进行排序的，但也利用了未选择的重组单倍体菌株。与染色体似乎为近端着丝粒的细胞学研究一致，在所研究的五个连锁群中，每个连锁群仅发现了一条臂。基于对从营养不亲和中逃逸的相反交配型菌株之间形成的二倍体的研究，交配型基因座matA已定位在连锁群I的tsgE-sprA区间内。
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