Francis D
Department of Biological Sciences, University of Delaware, Newark 19716, USA.
Genetics. 1998 Apr;148(4):1829-32. doi: 10.1093/genetics/148.4.1829.
Analysis of Dictyostelium development and cell biology has suffered from the lack of an ordinary genetic system whereby genes can be arranged in new combinations. Genetic exchange between two long ignored strains, A2Cycr and WS205 is here reexamined. Alleles which differ in size or restriction sites between these two strains were found for seven genes. Six of these are in two clusters on chromosome 2. Frequencies of recombinant progeny indicate that the genetic map of the two mating strains is colinear with the physical map recently worked out for the standard nonsexual strain, NC4. The rate of recombination is high, about 0.1% per kilobase in three different regions of chromosome 2. This value is comparable to rates found in yeast, and will permit fine dissection of the genome.
盘基网柄菌发育和细胞生物学的分析因缺乏一种能将基因排列成新组合的常规遗传系统而受到阻碍。这里重新审视了两个长期被忽视的菌株A2Cycr和WS205之间的基因交换。在这两个菌株之间发现了七个基因的等位基因，它们在大小或限制酶切位点上存在差异。其中六个基因位于2号染色体上的两个簇中。重组后代的频率表明，这两个交配菌株的遗传图谱与最近为标准无性菌株NC4绘制的物理图谱共线。重组率很高，在2号染色体的三个不同区域约为每千碱基0.1%。这个值与酵母中的重组率相当，将有助于对基因组进行精细剖析。
