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用金标法对大豆品种阿尔托纳的超薄切片进行大豆凝集素的超微结构定位。

Ultrastructural localization of soybean agglutinin on thin sections of Glycine max (soybean) var. Altona by the gold method.

作者信息

Horisberger M, Vonlanthen M

出版信息

Histochemistry. 1980 Feb;65(2):181-6. doi: 10.1007/BF00493167.

Abstract

Soybean agglutinin (SBA) has been localized in Glycine max (soybean) var. Altona at the ultrastructural level by the gold method. SBA was detected by marking thin sections of different part of the seed with gold granules (12 nm in size) labelled with anti-SBA antiserum. Upon examination by transmission electron microscopy, the lectin was found uniformly distributed in most of the protein bodies of the cotyledon. SBA was also present in the embryo axis.

摘要

大豆凝集素(SBA)已通过金标法在超微结构水平上定位到大豆品种阿尔托纳中。通过用抗SBA抗血清标记的金颗粒(大小为12纳米)标记种子不同部位的薄片来检测SBA。通过透射电子显微镜检查发现,凝集素均匀分布在子叶的大部分蛋白体中。SBA也存在于胚轴中。

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