Scaramuzzi R J, Baird D T, Clarke I J, Martensz N D, Van Look P F
J Reprod Fertil. 1980 Jan;58(1):27-35. doi: 10.1530/jrf.0.0580027.
Ewes were immunized against androstenedione-11 alpha-hemisuccinyl-bovine serum albumin (androstenedione-11-BSA) or BSA alone (controls). The ovaries were examined macroscopically and ovarian venous blood was collected at laparotomy by in-situ cannulation of the ovarian vein on Day 10 of the oestrous cycle, or by needle puncture of the ovarian vein 36 h after prostaglandin treatment. Follicular fluid was also collected from follicles greater than 5 mm diameter in some ewes. Ovaries and adrenal glands were collected and examined histologically. Oestrone, oestradiol-17 beta, androstenedione, testosterone and progesterone were measured by radioimmunoassays. Immunization against androstenedione-11-BSA was followed by an increase in the number of surface follicles greater than 3 mm in diameter and in ovulation rate. These changes were accompanied by an increased production of androgens (especially androstenedione) and of oestrogens during the preovulatory period. There were no observed changes in the adrenal cortex or pituitary glands, but the ovaries were heavier in immunized ewes and luteinized follicles were present in the ovaries of 1 of 4 immunized ewes. These results show that immunization against androstenedione-11-BSA leads to increased ovulation rate and suggest two possible mechanisms: (1) interference with the negative feedback effects of oestradiol-17 beta and/or (2) a reduction in the incidence of follicular atresia.
将母羊免疫于雄烯二酮 - 11α - 半琥珀酰 - 牛血清白蛋白（雄烯二酮 - 11 - BSA）或仅免疫牛血清白蛋白（作为对照）。在发情周期的第10天，通过卵巢静脉原位插管在剖腹术时宏观检查卵巢并收集卵巢静脉血，或者在前列腺素处理后36小时通过穿刺卵巢静脉收集血液。在一些母羊中，还从直径大于5毫米的卵泡中收集卵泡液。收集卵巢和肾上腺并进行组织学检查。通过放射免疫分析法测定雌酮、雌二醇 - 17β、雄烯二酮、睾酮和孕酮。用雄烯二酮 - 11 - BSA免疫后，直径大于3毫米的表面卵泡数量和排卵率增加。这些变化伴随着排卵前期雄激素（尤其是雄烯二酮）和雌激素产量的增加。未观察到肾上腺皮质或垂体有变化，但免疫母羊的卵巢较重，4只免疫母羊中有1只的卵巢存在黄体化卵泡。这些结果表明，用雄烯二酮 - 11 - BSA免疫可导致排卵率增加，并提示两种可能机制：（1）干扰雌二醇 - 17β的负反馈作用和/或（2）降低卵泡闭锁的发生率。
