一种从琼脂糖凝胶中分离纯DNA限制性片段的简便高效方法。

An easy and efficient procedure for the isolation of pure DNA restriction fragments from agarose gels.

作者信息

Ledeboer A M, Hille J, Schilperoort R A

出版信息

Biochim Biophys Acta. 1978 Oct 24;520(3):498-504. doi: 10.1016/0005-2787(78)90135-1.

Abstract

A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.

摘要

一种从琼脂糖凝胶中分离DNA的新方法被开发出来。利用一种印迹技术从凝胶中分离DNA。结果表明，分离出的DNA可用于限制性内切酶切割、大肠杆菌依赖DNA的RNA聚合酶合成互补RNA以及切口平移。该方法回收率高且易于操作。