Yogo Y, Furuno A, Nozawa A, Uchida S
J Virol. 1981 May;38(2):556-63. doi: 10.1128/JVI.38.2.556-563.1981.
We analyzed by blot hybridization the state and structure of the viral DNA in an exceptional BK virus-induced hamster tumor (choroid plexus papilloma Vn-324) that contains about one copy of the BK virus genome per cell, but no intranuclear T antigen as assayed by indirect immunofluorescence. The BK viral DNA was found to be integrated into cellular DNA at a site in the middle of the early region of the viral genome (between 0.32 and 0.41 map units). The structure of the inserted viral DNA shows that it cannot encode a full-size large T antigen, but may encode small T antigen and an N-terminal portion of large T antigen.
我们通过印迹杂交分析了一种特殊的BK病毒诱导的仓鼠肿瘤（脉络丛乳头状瘤Vn - 324）中病毒DNA的状态和结构。该肿瘤每个细胞含有约一个拷贝的BK病毒基因组，但通过间接免疫荧光检测未发现核内T抗原。发现BK病毒DNA整合到病毒基因组早期区域中部的一个位点的细胞DNA中（在0.32至0.41个图距单位之间）。插入的病毒DNA的结构表明它不能编码全长的大T抗原，但可能编码小T抗原和大T抗原的N端部分。
