Bourguignon L Y, Rozek R J
Cell Tissue Res. 1980;205(1):77-84. doi: 10.1007/BF00234444.
The distribution of ConA binding sites on the surface of normal human fibroblastoid cells grown in monolayer culture was carefully examined. Low concentrations of ConA (between 0.5-5.0 microgram/ml) were found to induce the ConA receptors to form a single, large cap structure. High concentrations of ConA (between 50-100 microgram/ml) inhibit cap formation at temperatures above 20 degrees C. Pretreatment of the cells in the cold or with colchicine allows cap formation to occur with high concentrations of ConA. The ConA caps appear to be preferentially localized near the nucleus. Using a double immunofluorescence technique, we have observed actin and myosin molecules concentrated underneath the surface receptor cap in the perinuclear region of the cells. These findings suggest that the binding of ConA to fibroblastoid cells may trigger the transmembrane association of cytoplasmic microfilaments with surface membrane receptors as previously proposed for lymphocytes and other round cells grown in suspension culture.
对单层培养的正常人成纤维样细胞表面伴刀豆球蛋白A（ConA）结合位点的分布进行了仔细研究。发现低浓度的ConA（0.5 - 5.0微克/毫升之间）可诱导ConA受体形成单个大的帽状结构。高浓度的ConA（50 - 100微克/毫升之间）在20摄氏度以上的温度下会抑制帽状结构的形成。在低温下或用秋水仙碱预处理细胞，可使高浓度的ConA形成帽状结构。ConA帽似乎优先定位于细胞核附近。使用双重免疫荧光技术，我们观察到肌动蛋白和肌球蛋白分子集中在细胞周核区域表面受体帽下方。这些发现表明，ConA与成纤维样细胞的结合可能会触发细胞质微丝与表面膜受体的跨膜结合，正如之前对悬浮培养的淋巴细胞和其他圆形细胞所提出的那样。
