Cornelis J J
Biochim Biophys Acta. 1978 Nov 21;521(1):134-43. doi: 10.1016/0005-2787(78)90256-3.
The elimination of cyclobutane pyrimidine dimers from the nuclear DNA of ultraviolet irradiated HeLa cells has been examined by means of chromatography and immunoautoradiography. The extent and duration of the process was similar when dimers were assayed by both methods, proving that the anti-sera recognized pyrimidine dimers. The rate of dimer excision did not differ through the cell cycle with the exception of mitosis during which no dimers were removed. Dimer excision is a relatively fast process which is terminated within a few hours, but it leaves many dimers in the DNA. Excision is depressed by inhibitors of semiconservative DNA synthesis that affect the DNA precursor pool or DNA polymerases. Cells whose DNA is partly substituted with bromodeoxyuridine instead of thymidine, repair single-strand breaks and remove dimers at the same rate but to different extents. On the other hand, inhibitors limit repair of breaks and removal of dimers to the same degree suggesting that the repair of the two types of lesion is coordinated.
通过色谱法和免疫放射自显影法研究了紫外线照射的HeLa细胞核DNA中环丁烷嘧啶二聚体的消除情况。当用两种方法检测二聚体时，该过程的程度和持续时间相似，证明抗血清识别嘧啶二聚体。除了有丝分裂期间不消除二聚体外，二聚体切除率在整个细胞周期中没有差异。二聚体切除是一个相对快速的过程，在几个小时内就会终止，但它会在DNA中留下许多二聚体。影响DNA前体池或DNA聚合酶的半保留DNA合成抑制剂会抑制切除。DNA部分被溴脱氧尿苷而非胸苷取代的细胞，以相同的速率但不同的程度修复单链断裂并去除二聚体。另一方面，抑制剂在相同程度上限制了断裂的修复和二聚体的去除，这表明这两种类型损伤的修复是协调的。
