Kuhn O, Tobler H
Biochim Biophys Acta. 1978 Nov 21;521(1):251-66. doi: 10.1016/0005-2787(78)90268-x.
Cold acid phenol extractions (Kirby, K.S. (1968) in Methods in Enzymology, Vol. XII B, pp. 87--99, Academic Press, New York) of homogenates from oocytes, zygotes and larval stages of Ascaris lumbricoides yield predominantly glycogen, some glycogen . phosphate complexes and guanine nucleotides, but only small amounts of RNA. Oocytes contain 10.0 pg of extractable 19 S and 26 S rRNA and 1.1 pg 4 S and 5 S RNA per cell. During the maturation of the oocyte to the zygote stage, the amount of 19 S and 26 S rRNA increases by 57%, and that of 4 S and 5 S RNA by 130%. Larval stages contain 62% more 19 S and 26 S rRNA, and 47% more 4 S and 5 S RNA than zygotes. The amount of the extractable glycogen decreases by about 80% and nucleotides by about 70% from oogenesis to the larval stage. The Kirby extract from homogenates of spermatids contains only traces of glycogen, glycogen . phosphate complexes and nucleotides, and 115 times less RNA per spermatid than oocytes. The nucleotide pool sizes of oocytes, zygotes and larval stages were determined; the pools consist of 71--88% guanine nucleotides. Spermatids contain only adenine nucleotides and an unidentified, nucleotide-like compound.
对蛔虫卵母细胞、受精卵和幼虫阶段的匀浆进行冷酸苯酚提取（柯比，K.S.（1968年），《酶学方法》，第十二卷B，第87 - 99页，学术出版社，纽约），主要得到糖原、一些糖原 - 磷酸复合物和鸟嘌呤核苷酸，但RNA含量很少。每个卵母细胞含有10.0皮克可提取的19 S和26 S核糖体RNA以及1.1皮克4 S和5 S RNA。在卵母细胞成熟为受精卵阶段的过程中，19 S和26 S核糖体RNA的量增加了57%，4 S和5 S RNA的量增加了130%。幼虫阶段的19 S和26 S核糖体RNA比受精卵多62%，4 S和5 S RNA比受精卵多47%。从卵子发生到幼虫阶段，可提取的糖原量减少了约80%，核苷酸量减少了约70%。精细胞匀浆的柯比提取物仅含有微量的糖原、糖原 - 磷酸复合物和核苷酸，每个精细胞的RNA含量比卵母细胞少115倍。测定了卵母细胞、受精卵和幼虫阶段的核苷酸库大小；这些库由71 - 88%的鸟嘌呤核苷酸组成。精细胞仅含有腺嘌呤核苷酸和一种未鉴定的类似核苷酸的化合物。
