17-羟孕酮和11-脱氧皮质醇的7α-和7β-羧甲基衍生物。合成与免疫原性

7 alpha- and 7 beta-carboxymethyl-derivatives of 17-hydroxyprogesterone and 11-deoxycortisol. Synthesis and immunogenic properties.

作者信息

Duval D, Prédine J, Emiliozzi R, Milgrom E

出版信息

Steroids. 1980 Jan;35(1):65-80. doi: 10.1016/0039-128x(80)90112-9.

Abstract

7 alpha- and 7 beta-carboxymethyl-derivatives of 17-hydroxyprogesterone and 11-deoxycortisol have been synthesized. After coupling to bovine serum albumin, they were used to elicit antibodies in rabbits. No major difference in the steroid specificity of the antisera was observed when either 7 alpha- or 7 beta-epimers were used for immunization. In both cases, highly specific antisera were obtained which may possibly be used to assay human plasma 17-hydroxyprogesterone and 11-deoxycortisol without chromatographic purification.

摘要

已合成了17-羟孕酮和11-脱氧皮质醇的7α-和7β-羧甲基衍生物。与牛血清白蛋白偶联后，用于在兔体内诱导产生抗体。当使用7α-或7β-差向异构体进行免疫时，未观察到抗血清的类固醇特异性有重大差异。在这两种情况下，均获得了高度特异性的抗血清，可能无需色谱纯化即可用于检测人血浆中的17-羟孕酮和11-脱氧皮质醇。