Kim S I, Charbonnier L, Mossé J
Biochim Biophys Acta. 1978 Nov 20;537(1):22-30. doi: 10.1016/0005-2795(78)90599-8.
The prolamin, avenin, was extracted from oat seeds and shown to be maximally extractable in 45% (w/w) ethanol. A purification procedure is described and some properties of avenin are examined. As with wheat and barley prolamins, salt soluble and glutelin fractions were simultaneously extracted. Starch gel electrophoresis revealed two novel fractions, which were isolated by ion-exchange chromatography. These fractions have similar amino acid composition, threonine as the N-terminal amino acid and both have a 22 500 molecular weight. It is suggested that the avenin constituents have a common ancestral gene.
醇溶谷蛋白燕麦蛋白从燕麦种子中提取出来，结果表明在45%（w/w）乙醇中其提取量最大。本文描述了一种纯化方法，并对燕麦蛋白的一些特性进行了研究。与小麦和大麦醇溶谷蛋白一样，盐溶级分和谷蛋白级分被同时提取出来。淀粉凝胶电泳显示出两个新级分，通过离子交换色谱法将它们分离出来。这些级分具有相似的氨基酸组成，苏氨酸为N端氨基酸，且二者分子量均为22500。有人提出燕麦蛋白成分具有一个共同的祖先基因。
