Belavtseva E M, Radchenko L G, Titova E F, Shalygin G F
Biofizika. 1980 May-Jun;25(3):405-8.
Comparative studies of some biological subjects with different water content (yeast cells, swollen starch grains, gels and gelatin and casein solutions) were conducted in scanning and transmitting electron electron microscope. The objects were prepared by cryogenic methods: freezing--drying; freezing--fracture, freezing--etching. It has been shown that when interpreting the micrographs of scanning microscopy for the systems biopolymer--water it is useful to apply the results of transmitting microscopy by using different procedures of preparation both with dehydration and without dehydration of the object. The electron micrographs have shown that with a rise in water content in the object the probability of artefact occurrence is increased for the objects studied in the scanning microscope by using freezing--drying method.
利用扫描电子显微镜和透射电子显微镜，对一些具有不同含水量的生物样本（酵母细胞、膨胀的淀粉颗粒、凝胶、明胶和酪蛋白溶液）进行了对比研究。这些样本采用低温方法制备：冷冻干燥、冷冻断裂、冷冻蚀刻。结果表明，在解释生物聚合物 - 水系统的扫描显微镜图像时，运用透射显微镜的结果会很有帮助，这可以通过对样本进行脱水和不脱水的不同制备程序来实现。电子显微镜图像显示，对于使用冷冻干燥法在扫描显微镜下研究的样本，随着样本含水量的增加，出现假象的概率也会增加。
