Heller J, Horácek V
Pflugers Arch. 1980 Jun;385(3):253-8. doi: 10.1007/BF00647465.
To gain access to glomeruli of the dog kidney, an approximately 0.3 mm thick superficial tissue layer was cut off from the convexity of the kidney. The surface was superfused with warm Ringer's. Renal perfusion pressure (PP) was changed by an aortic clamp. The afferent oncotic pressure (pi A) was calculated from arterial plasma protein concentration, the efferent (pi E) from pi A and single nephron filtration fraction. At PP 17 and 13kPa, directly measured GCP was significantly lower than that calculated from stop-flow pressure (SFP + pi A). At PP8kPa, the difference was not significant. Hydraulic pressure differences across the glomerular capillary wall was still significantly different from pi E at PP 17 and 13 kPa. Hence, filtration pressure equilibrium was achieved in the dog kidney only at PP 8kPa.
为了观察犬肾的肾小球，从肾凸面切下约0.3毫米厚的表层组织。肾表面用温热的林格氏液灌注。通过主动脉夹改变肾灌注压（PP）。根据动脉血浆蛋白浓度计算入球胶体渗透压（πA），根据πA和单肾单位滤过分数计算出球胶体渗透压（πE）。在PP为17和13kPa时，直接测量的肾小球毛细血管静水压（GCP）显著低于根据停流压力（SFP + πA）计算的值。在PP为8kPa时，差异不显著。在PP为17和13kPa时，肾小球毛细血管壁两侧的液压差仍与πE有显著差异。因此，犬肾仅在PP为8kPa时达到滤过压力平衡。
