Delaunay J, Fischer S, Tortolero M, Piau J P, Schapira G
Biomedicine. 1978 Jul;29(5):173-5.
We have investigated in human red blood cell membranes the presence of a 5'-nucleotidase, which is considered a general marker of plasma membranes. No activity was detectable with a highly sensitive colorimetric method. This absence is not due to the rapid decline of enzyme activity in the course of red cell ageing, nor to its release during membrane preparation; nor is it due to substrate accessibility hindrance to the active center of the enzyme. Thus, the ubiquitousness of 5'-nucleotidase in plasma membranes is questioned.
我们已经在人类红细胞膜中研究了5'-核苷酸酶的存在情况，该酶被认为是质膜的一个通用标志物。采用高灵敏度比色法未检测到活性。这种缺失并非由于红细胞衰老过程中酶活性的快速下降，也不是由于其在膜制备过程中的释放；也不是由于底物接近酶活性中心受到阻碍。因此，质膜中5'-核苷酸酶的普遍存在受到质疑。
