Petrov R V, Novikov V I, Zakharova L A, Stepanenko R N, Mikhaĭlova A A
Biull Eksp Biol Med. 1978 Nov;86(11):563-6.
An active factor doubling the antibody synthesis in the culture of immune lymph node cells was isolated from the supernatant of the cultures of bone marrow cells from intact donors. Gel chromatography was used for this purpose. The stimulant of antibody producers (SAP) was eluated at the site of cytochrome C exit; the factor is heat-stable and has the mol wt of 1300 daltons. SAP production needs RNA and protein synthesis, but does not require DNA synthesis. The radioactive label showed that SAP includes ribonucleotides and amino acids. It is suggested that SAP plays an important role in the regulation of antibody synthesis.
从健康供体骨髓细胞培养上清液中分离出一种能使免疫淋巴结细胞培养中抗体合成增加一倍的活性因子。为此采用了凝胶色谱法。抗体产生刺激因子（SAP）在细胞色素C洗脱部位被洗脱出来；该因子耐热，分子量为1300道尔顿。SAP的产生需要RNA和蛋白质合成，但不需要DNA合成。放射性标记显示SAP含有核糖核苷酸和氨基酸。提示SAP在抗体合成调节中起重要作用。
