Petrov R V, Mikhailova A A, Zakharova L A, Sergeyev J O, Novicov V I
Ann Immunol (Paris). 1980 Sep-Oct;131D(2):161-71.
The soluble factor recovered from supernatants of cultures of normal mouse bone marrow cells and previously isolated on Sephadex G-50 (n. w. 13,000 daltons), which stimulates antibody production when given at the peak of immune response, was further characterized in the present study. This stimulant of antibody producers (SAP) is active both in vitro and in vivo on PFC responses, and its effect does not show allogeneic or xenogeneic restriction. Using protein, RNA and DNA synthesis inhibitors, it was found that, in order to abolish the enhancing activity of treated bone marrow cells, protein synthesis must be inhibited by more than 80% and RNA synthesis by 20%, whereas inhibition of DNA synthesis by almost 100% did not interfere with the enhancing activity of treated cells. The stimulating activity of SAP was abolished by both RNase and pronase treatments, but heating at 100 degrees C for 10 min did not affect this activity. These properties indicate that SAP is a ribonucleoprotein complex. It is suggested that SAP mediates the regulatory function of bone marrow cells at the level of mature, differentiated cells of the B series.
从正常小鼠骨髓细胞培养上清液中回收的可溶性因子，先前在葡聚糖凝胶G - 50（净重13,000道尔顿）上进行过分离，在免疫反应高峰期给予时可刺激抗体产生，本研究对其进行了进一步表征。这种抗体产生刺激物（SAP）在体外和体内对空斑形成细胞（PFC）反应均有活性，且其作用不表现出同种异体或异种限制。使用蛋白质、RNA和DNA合成抑制剂发现，为了消除处理过的骨髓细胞的增强活性，蛋白质合成必须被抑制80%以上，RNA合成被抑制20%，而几乎100%抑制DNA合成并不干扰处理过的细胞的增强活性。SAP的刺激活性可被核糖核酸酶（RNase）和链霉蛋白酶处理消除，但在100℃加热10分钟并不影响该活性。这些特性表明SAP是一种核糖核蛋白复合物。有人提出，SAP在B系列成熟、分化细胞水平介导骨髓细胞的调节功能。
