离子强度对吖啶和喹啉衍生物的DNA-配体解旋角的影响。
The effect of ionic strength on DNA-ligand unwinding angles for acridine and quinoline derivatives.
作者信息
Jones R L, Lanier A C, Keel R A, Wilson W D
出版信息
Nucleic Acids Res. 1980 Apr 11;8(7):1613-24. doi: 10.1093/nar/8.7.1613.
Abstract
We have quantitatively examined the unwinding angles for the complexes of a related series of acridine and quinoline derivatives with DNA. Ethidium bromide was used as a control for determining superhelix densities at different ionic strengths. Relative to ethidium, 9-aminoacridine and quinacrine had an essentially constant unwinding angle of approximately 17 degrees at all ionic strengths tested. The apparent unwinding angle for chloroquine and 9-amino-1,2,3,4-tetrahydroacridine was found to be ionic strength dependent, increasing with increasing ionic strength. This suggests that competitive nonintercalative binding at low ionic strengths causes an apparent lowering of the quinoline unwinding angle. This can also explain why 4-aminoquinaldine, examined at low ionic strength, gives a quite low apparent unwinding angle. Quinacrine along with chloroquinine and 9-aminoacridine approaches a limiting value for their unwinding angle of approximately 17 degrees. 4-aminoquinaldine and 9-amino-1,2,3,4-tetrahydroacridine could not be examined at an ionic strength above 0.03 because of their very low equilibrium binding constants.
摘要
我们已经定量研究了一系列相关的吖啶和喹啉衍生物与DNA形成的复合物的解旋角度。溴化乙锭用作对照，以测定不同离子强度下的超螺旋密度。相对于溴化乙锭，在所有测试的离子强度下，9-氨基吖啶和喹吖因的解旋角度基本恒定，约为17度。发现氯喹和9-氨基-1,2,3,4-四氢吖啶的表观解旋角度与离子强度有关，随离子强度增加而增大。这表明在低离子强度下竞争性的非嵌入结合导致喹啉解旋角度明显降低。这也可以解释为什么在低离子强度下检测的4-氨基喹哪啶的表观解旋角度相当低。喹吖因与氯喹和9-氨基吖啶的解旋角度接近约17度的极限值。由于4-氨基喹哪啶和9-氨基-1,2,3,4-四氢吖啶的平衡结合常数非常低，无法在离子强度高于0.03的条件下进行检测。
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