快速灵敏测定β-内酰胺类抗生素的酶法
Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics.
作者信息
Frère J M, Klein D, Ghuysen J M
出版信息
Antimicrob Agents Chemother. 1980 Oct;18(4):506-10. doi: 10.1128/AAC.18.4.506.
Abstract
A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk.
摘要
本文描述了一种快速灵敏的β-内酰胺类抗生素估算方法，该方法利用了这些抗生素使R39 D-羧肽酶失活的能力。根据控制反应的动力学参数值，抗生素可分为两组。I组和II组抗生素定量估算的下限分别约为5皮摩尔/毫升和50皮摩尔/毫升。该方法已应用于人体血清和牛奶等生物流体。
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本文引用的文献
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Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.链霉菌R39胞外DD-羧肽酶-转肽酶的分子量、氨基酸组成及理化性质
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