β-内酰胺抗生素与来自链霉菌R39的细胞外DD-羧肽酶-转肽酶之间的相互作用模式
Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.
作者信息
Fuad N, Frère J M, Ghuysen J M, Duez C, Iwatsubo M
出版信息
Biochem J. 1976 Jun 1;155(3):623-9. doi: 10.1042/bj1550623.
Abstract
The exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39 is inhibited by beta-lactam antibiotics according to the same general scheme of reaction as the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61. However, the values for the kinetic constants involved in the reaction are very different for the two enzymes and provide an explanation for the observation that the R39 enzyme is more sensitive to beta-lactam antibiotics than the R61 enzyme. Further, particular beta-lactams influence the kinetic constants to different extents depending on the source of the enzyme, so that a physical basis for the spectrum of antibiotic activity against particular enzyme systems is provided.
摘要
根据与链霉菌R61的胞外DD-羧肽酶-转肽酶相同的一般反应模式，链霉菌R39的胞外DD-羧肽酶-转肽酶受到β-内酰胺抗生素的抑制。然而，两种酶参与反应的动力学常数的值差异很大，这就解释了为什么R39酶比R61酶对β-内酰胺抗生素更敏感。此外，特定的β-内酰胺根据酶的来源不同程度地影响动力学常数，从而为针对特定酶系统的抗生素活性谱提供了物理基础。
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