大鼠肝脏质膜在再生过程中的流动性变化。

Fluidity changes in rat liver plasma membranes during regeneration.

作者信息

Mazzanti L, Curatola G, Lenaz G, Trentalance A

出版信息

Boll Soc Ital Biol Sper. 1980 Jun 15;56(11):1114-8.

PMID:7192562

Abstract

The fluidity of the liver plasma membrane during the first cell cycle of hepatectomized rats has been investigated by means of stearic acid spin labels and of the fluorescence polarization of perylene. The order parameters of 5-doxylstearate, that probes the membrane surface, have been found lowered in the hepatectomized rats after 15 hours from operation, in comparison with anesthetised and sham-operated controls at the same time; on the contrary, the fluidity in the membrane core, as probed by 16-doxylstearate, is increased in all groups. The fluorescence polarization of perylene is also lower in the hepatectomized rats. The results suggest that liver regeneration is accompanied by a fluidity increase of the plasma membrane.

摘要

利用硬脂酸自旋标记物和苝的荧光偏振，研究了肝切除大鼠第一个细胞周期中肝细胞质膜的流动性。与同时的麻醉对照组和假手术对照组相比，在术后15小时，探测膜表面的5-脱氧硬脂酸盐的序参数在肝切除大鼠中降低；相反，用16-脱氧硬脂酸盐探测的膜核心中的流动性在所有组中均增加。肝切除大鼠中苝的荧光偏振也较低。结果表明，肝再生伴随着质膜流动性的增加。