Poste G
Prog Clin Biol Res. 1980;41:737-45.
B16 mouse melanoma sublines cultured in vitro spontaneously shed intact vesicles of plasma membrane. These vesicles can be fused with the plasma membrane of cells from homologous and heterologous B16 sublines using polyethylene glycol (PEG) and phytohemagglutinin (PHA). The ability of FI cells to arrest in the lung and form metastases in this organ is significantly increased by fusion of vesicles from a highly metastatic subline (F10) that localizes exclusively in the lung with cells from another subline (F1) which is poorly metastatic and produce few lung metastases. In contrast, fusion of F1 vesicles with F10 cells does not reduce their ability to localize in the lung of form lung metastases. Vesicle-treated F1 cells revert to their original arrest behavior and metastatic capacity following removal of F10 vesicle components from the plasma membrane. The changes in the arrest and metastatic behavior of F1 cells induced by F10 vesicles are highly specific. Vesicles from other B16 sublines which show limited abilities to localize in the lung (F1, F1(1r) and F10(1r) fail to modify the arrest behavior of F1 cells. These results suggest that the differences in the ability of the F1 and F10 sublines to localize in the lung are determined by differences in surface properties.
体外培养的B16小鼠黑色素瘤亚系能自发脱落完整的质膜囊泡。使用聚乙二醇（PEG）和植物血凝素（PHA），这些囊泡可与同源和异源B16亚系细胞的质膜融合。通过将仅定位于肺部的高转移性亚系（F10）的囊泡与转移性差且肺部转移灶少的另一亚系（F1）的细胞融合，F1细胞在肺部停滞并在该器官形成转移灶的能力显著增强。相反，F1囊泡与F10细胞融合并不会降低其在肺部定位或形成肺转移灶的能力。从质膜上去除F10囊泡成分后，经囊泡处理的F1细胞恢复其原始的停滞行为和转移能力。F10囊泡诱导的F1细胞停滞和转移行为的变化具有高度特异性。其他在肺部定位能力有限的B16亚系（F1、F1(1r)和F10(1r)）的囊泡无法改变F1细胞的停滞行为。这些结果表明，F1和F10亚系在肺部定位能力上的差异是由表面性质的差异决定的。
