Poste G, Nicolson G L
Proc Natl Acad Sci U S A. 1980 Jan;77(1):399-403. doi: 10.1073/pnas.77.1.399.
B16 mouse melanoma sublines in culture spontaneously shed intact plasma membrane vesicles. These vesicles can be fused with the plasma membrane of cells from homologous and heterologous B16 sublines by using polyethylene glycol and phytohemagglutinin-P. Fusion of vesicles from a highly metastatic subline (F10) that localizes exclusively in the lung with cells from a poorly metastatic subline (F1) significantly increased the ability of F1 cells to become arrested in the lung and form metastases in this organ. In contrast, fusion of F1 vesicles with F10 cells did not alter the ability of vesicle-modified cells to localize in the lung or form lung metastases. F10 vesicle-modified F1 cells reverted to their original arrest behavior and metastatic capacity after removal of F10 vesicle components from the plasma membrane. The changes in the arrest and metastatic behavior of F10 vesicle-modified F1 cells were highly highly specific. Vesicles from other B16 sublines that are poorly metastatic and show limited localization in the lung (F1, FLLr, and F10Lr) did not modify the arrest behavior and metastatic capacity of FU cells. These results suggest that the differences in the abilities of the F1 and F10 sublines to localize in the lung are determined by differences in cell surface properties.
培养中的B16小鼠黑色素瘤亚系能自发脱落完整的质膜囊泡。使用聚乙二醇和植物血凝素-P，这些囊泡可与同源和异源B16亚系细胞的质膜融合。来自高度转移性亚系（F10）且仅定位于肺部的囊泡与转移性较差的亚系（F1）细胞融合，显著提高了F1细胞在肺部滞留并在该器官形成转移灶的能力。相反，F1囊泡与F10细胞融合并未改变囊泡修饰细胞在肺部定位或形成肺转移灶的能力。从质膜上去除F10囊泡成分后，F10囊泡修饰的F1细胞恢复了其原始的滞留行为和转移能力。F10囊泡修饰的F1细胞的滞留和转移行为变化具有高度特异性。来自其他转移性较差且在肺部定位有限的B16亚系（F1、FLLr和F10Lr）的囊泡并未改变FU细胞的滞留行为和转移能力。这些结果表明，F1和F10亚系在肺部定位能力上的差异是由细胞表面特性的差异决定的。
