Alonso R, Ozaki Y, Wurtman R J
Reproduccion. 1980 Jul-Sep;4(3):225-33.
The levels of melatonin in urine, serum, and pineals were determined in rats during the light/dark cycle. Previous to the radioimmunoassay, melatonin was isolated using a new ascending chromatographic method with programmed multiple development. The daily excretion of melatonin and its serum levels showed a characteristic rhythm, night samples containing several times more melatonin than those of the light period. The pineal content of melatonin of animals killed at different intervals also showed a similar day/night rhythm. The chromatographic method used gave a perfect separation of melatonin from other materials which could interfere in the assay. The mobility of material detected by RIA was identical to that of authentic melatonin chromatographed concurrently.
在光照/黑暗周期中测定了大鼠尿液、血清和松果体中的褪黑素水平。在放射免疫测定之前，采用一种新的程序多次展开的上行色谱法分离褪黑素。褪黑素的每日排泄量及其血清水平呈现出特征性节律，夜间样本中的褪黑素含量比白天样本高几倍。在不同时间间隔处死的动物的松果体褪黑素含量也呈现出类似的昼夜节律。所使用的色谱方法能将褪黑素与其他可能干扰测定的物质完美分离。放射免疫分析法检测到的物质的迁移率与同时进行色谱分析的纯褪黑素的迁移率相同。
