Ozaki Y, Lynch H J, Wurtman R J
Endocrinology. 1976 Jun;98(6):1418-24. doi: 10.1210/endo-98-6-1418.
The concentrations of melatonin in rat pineals and in samples of rat plasma and urine were measured by bioassay or radioimmunoassay. Melatonin excretion during the daily dark period (0.98 +/- 0.07 ng/12 h, by bioassay; 1.40 +/- 0.08 ng/12 h, by radioimmunoassay) was much greater than during the light period (0.18 +/- 0.04 ng/12 h, by bioassay; 0.40 +/- 0.04 ng/12 h by radioimmunoassay). (The radioimmunoassay is not completely specific when applied to materials extracted from utine.) Pineal glands and plasmas obtained from animals killed during the daily dark period also contained much more melatonin than samples from animals killed during the light period. Pretreatment with chlorpromazine slowed the disappearance of exogenous melatonin from rat plasma and markedly raised the concentrations of endogenous melatonin in both the pineal gland and the plasma.
通过生物测定法或放射免疫测定法测量大鼠松果体、大鼠血浆和尿液样本中褪黑素的浓度。每日黑暗期的褪黑素排泄量（生物测定法为0.98±0.07纳克/12小时；放射免疫测定法为1.40±0.08纳克/12小时）远高于光照期（生物测定法为0.18±0.04纳克/12小时；放射免疫测定法为0.40±0.04纳克/12小时）。（当应用于从尿液中提取的物质时，放射免疫测定法并非完全特异。）从每日黑暗期处死的动物获得的松果体和血浆中所含的褪黑素也比光照期处死的动物样本多得多。用氯丙嗪预处理可减缓外源性褪黑素从大鼠血浆中的消失，并显著提高松果体和血浆中内源性褪黑素的浓度。
